Development and Validation of a Method Using Dried Oral Fluid Spot to Determine Drugs of Abuse

A liquid chromatography–mass spectrometry method using dried oral fluid spots was developed and validated for the simultaneous quantification of cocaine, benzoylecgonine, cocaethylene, amphetamine, and 3,4‐methylenedioxymethamphetamine. The oral fluid was applied to a Whatman 903 grade paper and submitted to a drying time of 2.5 h. The extraction procedure was optimized by chemometric approach using simplex centroid design. Spots were extracted with a mixture of acetonitrile, buffer, and methanol. Calibration curves covered a linear concentration range of 40–500 ng/mL. Validation parameters of linearity, precision, accuracy, selectivity, carryover, matrix effects, and stability were evaluated and showed satisfactory results. Spot homogeneity was also satisfactory, with less than 15% of deviation from nominal concentration. Spot volume did not influence accuracy when less than 100 μL of the sample was applied to the spot. The validation of the proposed method suggests a potential application in different scenarios in toxicology.     

Governance Arrangements for Transboundary Issues: Lessons from the Food Waste Programs of Italian Regions

Focusing on transboundary issues contributes to highlighting how new governance modes can emerge from increasing interdependence and complexity in public policies. This article analyzes food waste as a transboundary issue that cuts across different policy subsystems (PSs), intersects multiple levels of government, and calls into question consolidated policy making for new modes of governance. The analytical framework provides a multidimensional approach and a new typology of governance arrangements to be used in empirical research. Evidence from an Italian case study and an empirical investigation of 20 regional food waste programs shows that regions can adopt different driving ideas and can activate a variety of governance arrangements and differentiated, as well as viable, modes of integration between consolidated PSs. The article argues that different tool mixes combined with different levels of governance complexity may lead to a variety of governance arrangements and a differentiated likelihood of policy integration.     

Building Codes and Energy Efficiency: How New York City's Building Codes Succeed and Fail in Regulating GHG Emissions

This article seeks to analyze how effective the local NYC building codes are in promoting energy efficiency and regulating greenhouse gas emissions. Part I will highlight the importance of building efficiency and set the stage for how these changes can affect the global climate. Part II will look at specific NYC programs and agencies that are tasked with monitoring this issue, and what their reports indicate as progress in this area. Part III will consider whether what is currently being done is sufficient, and how we might better achieve NYC's emission reduction goals in the building efficiency sector.     

Application of capillary electrophoresis to examination of color inkjet printing inks for forensic purposes

In the process of questioned document examination, the discrimination of inkjet printing inks is becoming more necessary due to increasingly frequent counterfeiting of documents printed by inkjet printers. Therefore, a method based on micellar electrophoretic capillary chromatography (MECC) has been developed and applied to analysis of such inks extracted from paper. With the use of an optimized and validated analytical procedure, multielectropherograms of inks taken from various models of printers made by various producers (Hewlett-Packard, Epson, Brother, Lexmark and Canon) were created. It was shown that effective differentiation of individual inks was possible in terms of migration time, order and specific shapes of characteristic peaks. By comparison of recorded UV-Vis spectra, the identification of main dyes was also achievable. The usefulness of the method was confirmed by an intralaboratory test of utility, in which several forged printouts were successfully examined. The obtained results proved that the proposed procedure is a useful tool that could be applied to ink discrimination and group identification of dyes originating from inkjet printing inks. Consequently, the developed method can be applied in the forensic field, including investigation of the authenticity of documents.     

Wife-battering — An Australian perspective

This article examines the leading definitions of wife-battering and questions the emphasis on physical rather than nonphysical abuse. All dimensions of these definitions are analyzed, namely the form of abuse, severity, frequency, intention, and meaning. The final definition formulated is derived from these dimensions and from the accounts and experiences of battered women themselves. The incidence and etiology of wife-battering is then examined from an Australian perspective. Theories which concentrate either specifically on the profiles of the victim and perpetrator or on the family unit or external factors are discussed. Finally, women do remain in violent relationships and their reasons for doing so are considered.     

Risky Business: What Happens to Gender Equality and Women's Rights in Post-Conflict Societies? Insights from NGO's in El Salvador

Using the case of El Salvador, this paper explores how women's organizational skills developed in civil war translate into work in NGOs in the post-conflict struggle for rights. The paper briefly describes the gender stratification methodology used in the analysis and then presents the situation in El Salvador before, during, and after the war. After discussing how Salvadoran women, despite quite limited economic power, became a well-organized force that was strategically indispensable to the rebels during the war, the paper examines factors that contributed to the success of Las Madres Demandantes (LMD), an NGO focused on the single issue of getting child support payments to women. The experience of other NGOs in El Salvador is reviewed with respect to the factors that contributed to the success of LMD. In conclusion, a few lessons from the issues faced by the post-conflict women's NGOs in El Salvador are presented.     

Sequence analysis of a new short tandem repeat locus D17S2266E located in the human growth hormone gene cluster HumGH@

D17S2266E is a new, variable genetic marker exhibiting polymorphism of the number of repeats of four- and two-nucleotide motifs. This study, carried out on a group of 250 unrelated persons from various regions of Poland, revealed the presence of 24 different alleles ranging in size from 232 to 290 base pairs. Analysis of the sequenced fragments demonstrated that the alleles consisted of two flanking regions and two variable blocks that were separated by a consensus sequence. There were (AAAG)(5)(AG)(1)(AAAG)(3-4) repeats in the first block, and [(AAAG)(2)(AG)(1)](0-1)[(AAAG)(6)(AG)(1)](0-1)(AAAG)(n) repeats in the second block. On the basis of the allele frequencies in the population, we were able to do biostatistical calculations, which gave the following results: expected heterozygosity 0.8947 +/- 0.0137, power of discrimination 0.9793, polymorphism information content 0.8837, probability of exclusion (PE) 0.7859, PE for motherless cases 0.6473, and an average paternity index of 4.7470. These biostatistical parameters show that the marker D17S2266E can find a wide range of applications in forensic testing.     

An application of Gough's conceptual model to a measure of adolescent self-esteem

The concept of self-esteem is central in psychology, but few well-validated measures exist. This paper presents some preliminary data on the Self-Esteem Questionnaire (SEQ) organized along Gough's conceptual model of primary, secondary, and tertiary evaluation. The SEQ appears to discriminate groups along a self-esteem continuum; fits in well with a variety of theoretical approaches; does not have nuisance loadings on sex, intelligence, or social status; is unduly affected by social desirability; and correlates well with standard measures of self-esteem.Received his Ph.D. in Clinical Psychology from the University of California, Berkeley. Research interests include psychological testing and creativity.Received her B.A. from the University of Arizona. Research interests include child abuse and statistical methodology.